Azocoll-digesting Proteinases in Soybean Leaves
نویسندگان
چکیده
منابع مشابه
Hemoglobin-digesting Acid Proteinases in Soybean Leaves: CHARACTERISTICS AND CHANGES DURING LEAF MATURATION AND SENESCENCE.
Three proteinases which digest hemoglobin rapidly at acid pH (3.5 to 4.5) were identified in crude extracts of soybean (Merr.) leaves and separated by chromatography on DEAE-cellulose. All three enzymes were endopeptidases as judged by the ratio of alpha-amino-nitrogen plus peptide nitrogen over alpha-amino-nitrogen in the trichloroacetic acid-soluble portion of hemoglobin digests. Proteinase I...
متن کاملAssaying proteinases with azocoll.
Azocoll, an insoluble, ground collagen to which a bright-red azodye is attached has been widely used for the assay of proteolytic enzymes. Earlier studies showed that hydrolysis of azocoll progressed linearly as a function of proteinase concentration but in an exponentially increasing manner as a function of time. No explanation for the latter behavior has been offered. We have found that assay...
متن کاملCell Walls of Phaseolus vulgaris Leaves Contain the Azocoll-Digesting Proteinase.
The leaves of Phaseolus vulgaris L. cv Greensleeves contain an endopeptidase with a pH optimum of 9.0 and an isoelectric point between 10.0 and 10.5. This endopeptidase is the only abundant Azocoll-digesting proteinase in the leaves. The activity of this enzyme is highest in immature leaves and declines as the leaf matures and senesces. Enzymically isolated protoplasts contain very little of th...
متن کاملSoybean leaves contain multiple lipoxygenases.
Chromatofocusing of soybean (Glycine max L.) leaf lipoxygenases revealed three distinct peaks of activity. Based on their isoelectric points (pls), pH optima, and mutant analysis it appears that the leaf isozymes are different from those described from mature soybean seed. At least one leaf lipoxygenase appears to differ from those found in hypocotyls. The pls of the main bands of the three lea...
متن کاملEstimation of Protein Turnover in Soybean Leaves
Magic angle cross-polarization 16N nuclear magnetic resonance spectra of intact lyophilized soybean leaves have been obtained at 9.12 MHz. The leaves were harvested over a 6-week period fr m "N-uniformly labeled plants exposed to 13C02 for 1 to 7 days. The concentration of 13C-16N double labels in the main chains of leaf protein was determined nondestructively by double cross-polarization techn...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.64.5.857